SUMO Protease (His-tagged) is a fusion protein with a His tag added to the N-terminus of the SUMO Protease amino acid sequence. It is expressed as a fusion protein in E. coli and purified through multiple chromatography methods to achieve highly purified fusion protein. The optimal reaction temperature for this enzyme is 30°C, but it maintains high cleavage activity under a range of conditions (4~30°C, pH 7.5~9, 0~400mM NaCl, etc.). The fused His tag facilitates a removal of the enzyme using IMAC affinity chromatography after cleavage of the target protein.

Sizes：10KU, 50KU, 100KU, 500KU
Mol. Wt.：27.8 kDa
Theory pI：6.34
Specific Activity：≥500 U/μl
Resource：Escherichia coli (E. coli)
Purity：≥90% as determined by SDS-PAGE
Quantitative Method：UV absorption, extinction coefficient E (0.1% at 280 nm) = 1.026
Storage Conditions：Stored at -80°C
Storage Duration：Stored at -80°C for one year and at -20°C for 6 months