SUMO Protease 1 (GST-tagged) is a fusion protein with a GST tag added to the N-terminus of the SUMO Protease 1 amino acid sequence. It is expressed as a fusion protein in E. coli and purified through multiple chromatography methods to achieve highly purified fusion protein. The optimal reaction temperature for this enzyme is 30°C, but it maintains high cleavage activity under a range of conditions (4~30°C, pH 7~9, 0~300mM NaCl, etc.). The fused GST tag facilitates a removal of the enzyme using GST affinity chromatography after cleavage of the target protein.

Sizes：5KU, 10KU, 50KU
Mol. Wt.：52.3 kDa
Theory pI：5.46
Specific Activity：≥50 U/μl
Resource：Escherichia coli (E. coli)
Purity：≥85% as determined by SDS-PAGE
Quantitative Method：UV absorption, extinction coefficient E (0.1% at 280 nm) = 1.364
Storage Conditions：-80°C
Storage Duration：Stored at -80°C for one year and at -20°C for 6 months