The rProtein A ligand is expressed in E. coli, ensuring that it is free from animal-derived components while retaining all five binding domains of the wild-type protein.
ProA NUPharose FF exhibits significantly higher binding capacity compared to wild-type Protein A resins, with a dynamic binding capacity for human IgG exceeding 40 mg/mL.
This resin demonstrates affinity towards various types and subtypes of antibodies from multiple species, including human, mouse, rat, rabbit, and bovine sources.
It is capable of isolating and purifying different subclasses of mammalian antibodies or genetically engineered recombinant proteins containing Fc fragments from ascites, serum, cell culture supernatants, or cell lysates, thereby meeting research and production requirements at various scales.