Phenyl NUPharose FF and Phenyl NUPharose FF (LS) are hydrophobic chromatography media formed by coupling phenyl groups to agarose gel microspheres via stable ether bonds. The phenyl group is strongly hydrophobic and exhibits hydrophobic and π-π interactions with aromatic amino acid residues, making it suitable for target protein capture and intermediate purification.
Phenyl NUPharose FF has a higher ligand density than Phenyl NUPharose FF(LS), resulting in different hydrophobicity and selectivity towards samples. Users can choose the appropriate product model based on specific application scenarios.
Phenyl NUPharose HP is a high-resolution phenyl resin (HP stands for High Performance). Phenyl NUPharose HP features high resolution and is typically used in the polishing stage. Typical applications of phenyl resins include the production of recombinant human granulocytemacrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (GCSF), human macrophage colony-stimulating factor (M-CSF), epidermal growth factor (EGF), and the purification of monoclonal antibodies.