The NUPhadex series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. Dextran exhibits extremely low non-specific adsorption to biomolecules.
These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules
in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids. The globular protein exclusion limits increase progressively for NUPhadex G-75 M, NUPhadex G-100 M, NUPhadex G-150 M, and NUPhadex G-200 M. These four products are not rigid microspheres; the column bed is compressible and they are typically used with gravity flow.