The NUPhadex G-10 M, NUPhadex G-15 M, and NUPhadex G-50 M series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. The dry powder particle sizes of these three products are similar. After swelling, the microspheres are rigid, with decreasing crosslinking density, increasing swelling factor for the dry powder, and increasing globular protein exclusion limits in the order G-10 M < G-15 M < G-50 M.
Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product series suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids.