Compared to its corresponding column chromatography agarose resin (Ni-NTA NUPharose FF, this product features the same ligand modification process and maintains a good balance between affinity (capacity) and specificity (selectivity):
while the binding capacity for most His-tagged proteins exceeds 40 mg/mL, sample purity can reach over 90% after a single purification step, with low Ni2+ leakage in the eluate (Ni2+ to target protein molar ratio <0.1).
The tetra-coordinate structure formed by the NTA ligand and Ni2+ in this product is stable. After chelation, it can tolerate certain concentrations of chelating agents, reducing agents, and alkali. When samples contain 1 mM EDTA, 1~10 mM β-mercaptoethanol, or 5 mM DTT, purification can proceed normally with Ni-NTA NUPharose Mag, followed by cleaning with 0.1~1 M NaOH.