NHS Activated NUPharose Mag is a pre-activated magnetic resin product. The surface of the paramagnetic agarose gel microsphere matrix is modified with a ten-atom-long spacer arm, followed by activation of the carboxyl groups with N-hydroxysuccinimide (NHS). The NHS-activated groups can couple with functional biomacromolecules containing primary amines, such as antibodies, proteins, enzymes, and peptides, to form stable amide bonds.
The resulting affinity magnetic beads can specifically bind target molecules, allowing high-purity product isolation in a single purification step.
Compared to column chromatography with agarose resins, with below advantages:
* Clarification of the feedstock is generally not required, shortening purification time and offering better enrichment effects for low-expression feedstocks;
* Both manual and automated batch operations are possible. Manual operation is equipment-independent and offers high operational throughput.