Chromatography resin - Dotway Biotech -- Mfg for recombinant protein and chromatography resin

NUPhadex G-10M, 15M & 50M Size Exclusion Resin

The NUPhadex G-10 M, NUPhadex G-15 M, and NUPhadex G-50 M series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. The dry powder particle sizes of these three products are similar. After swelling, the microspheres are rigid, with decreasing crosslinking density, increasing swelling factor for the dry powder, and increasing globular protein exclusion limits in the order G-10 M < G-15 M < G-50 M. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product series suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids.

NUPhadex G-75M, 100M, 150M and 200M Size Exclusion Resin

The NUPhadex series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids. The globular protein exclusion limits increase progressively for NUPhadex G-75 M, NUPhadex G-100 M, NUPhadex G-150 M, and NUPhadex G-200 M. These four products are not rigid microspheres; the column bed is compressible and they are typically used with gravity flow.

NUPhadex G-25 Size Exclusion Resin

The NUPhadex G-25 series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. These products have an exclusion limit of approximately 5000 Da for globular proteins. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids. It can also be used for fractionation processes to separate and purify biomolecules with small molecular weight differences, such as peptides, oligonucleotides, antibiotics, and natural products.

NuPore 30, NuPore 75 and NuPore 200 Size Exclusion Resin

The NuPore series products are high-performance size exclusion resins formed by coupling dextran to a cross-linked agarose base matrix. These composite resin microspheres have a mean particle size of approximately 35 μm, combining the excellent mechanical properties of agarose with the high selectivity of the dextran network. Compared to traditional size exclusion resins, the NuPore series offers advantages such as high rigidity, high resolution, and high flow rates. They can be used for group separations like desalting and buffer exchange, as well as for fractionation processes of biomacromolecules such as peptides, proteins, nucleic acids with similar molecular weights, and components of natural products.

4FF and 6FF Gel Filtration Resin

NUPharose 4FF and NUPharose 6FF are agarose-based gel filtration resins from our classic NUPharose technology platform. Gel filtration resins are also referred to as size exclusion resins. NUPharose 4FF and NUPharose 6FF consist of 4% and 6% crosslinked agarose, respectively, with unique pore sizes and pore size distributions. Thanks to strict raw material screening and control, as well as innovations in bead forming and cross-linking processes, these products exhibit excellent pressure/flow characteristics and low non-specific adsorption to biomacromolecules, resulting in high product recovery. They are particularly suitable for the separation of larger particles such as viral particles and exosomes. Furthermore, NUPharose 4FF and NUPharose 6FF possess good physical and chemical stability. They can serve as base matrices and, after modification with specific small molecule or macromolecule ligands, can be used in various bioseparation processes.

4B and 6B Gel Filtration Resin

NUPharose 4B and NUPharose 6B are agarose-based gel filtration resins from the NUPharose technology platform. Gel filtration resins are also referred to as size exclusion resins. NUPharose 4B and NUPharose 6B consist of 4% and 6% agarose, respectively. Different concentrations of agarose solutions form different pore sizes upon gelation. The refined agarose has charged impurities removed, resulting in very low non-specific adsorption. These products are suitable for separating biomolecules of various molecular weights. Additionally, NUPharose 4B and NUPharose 6B can be activated for coupling different affinity ligands. we also offer epoxy-activated NUPharose 6B (Epoxy Activated NUPharose 6B).

Butyl Hydrophobic Resin

Butyl NUPharose FF is a hydrophobic chromatography medium with moderately strong hydrophobicity, formed by coupling n-butyl groups to agarose gel microspheres via stable ether bonds. Butyl NUPharose HP is a high-resolution butyl resin (HP stands for High Performance), which features high resolution and is typically used in the polishing stage. The n-butyl group is a “pure hydrophobic” group and exhibits selectivity differences towards various hydrophobic amino acid residues compared to phenyl groups. This product retains the excellent hydrophilicity and large network structure of agarose, offering good compatibility with bioactive macromolecules, high capacity, low non-specific adsorption, and high flow rates. It is widely used for laboratory-scale preparation of biomacromolecules such as proteins and peptides with certain hydrophobic properties, as well as for industrial-scale production in biopharmaceuticals and bioengineering.

Phenyl Hydrophobic Resin

Phenyl NUPharose FF and Phenyl NUPharose FF (LS) are hydrophobic chromatography media formed by coupling phenyl groups to agarose gel microspheres via stable ether bonds. The phenyl group is strongly hydrophobic and exhibits hydrophobic and π-π interactions with aromatic amino acid residues, making it suitable for target protein capture and intermediate purification. Phenyl NUPharose FF has a higher ligand density than Phenyl NUPharose FF(LS), resulting in different hydrophobicity and selectivity towards samples. Users can choose the appropriate product model based on specific application scenarios. Phenyl NUPharose HP is a high-resolution phenyl resin (HP stands for High Performance). Phenyl NUPharose HP features high resolution and is typically used in the polishing stage. Typical applications of phenyl resins include the production of recombinant human granulocytemacrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (GCSF), human macrophage colony-stimulating factor (M-CSF), epidermal growth factor (EGF), and the purification of monoclonal antibodies.

CM Ion Exchange Resin

Carboxymethyl (CM) is a weak cation exchanger. Compared to strong cation exchange (SP) media, weak cation exchange media offer different selectivity and can be considered when SP media fail to provide the required selectivity. Based on resolution and application, the CM NUPharose series can be divided into two products: CM NUPharose FF (~90 μm) and CM NUPharose HP (~35 μm). FF stands for Fast Flow. CM NUPharose FF features fast flow rates, suitable for rapid protein purification, wide applicability, and ease of scale-up. HP stands for High Performance. CM NUPharose HP offers high resolution and is often used in polishing stages.

DEAE Ion Exchange Resin

The DEAE NUPharose series are weak anion exchange resins, where DEAE stands for Diethylaminoethyl. Compared to strong anion exchange (Q) resins, weak anion exchange resins offer different selectivity and can be considered when Q resins do not meet selectivity requirements. Based on resolution and application scenarios, the DEAE NUPharose series can be divided into two products: DEAE NUPharose FF (~90 μm) and DEAE NUPharose HP (~35 μm). FF stands for Fast Flow; DEAE NUPharose FF features fast flow rates suitable for rapid protein purification, wide applicability, and easy scale-up. HP stands for High Performance; DEAE NUPharose HP offers high resolution and is often used in polishing stages.

Co-NTA Metal Chelate Affinity Resin

Co-NTA NUPharose FF is a metal chelate resin containing nitrilotriacetic acid (NTA) groups, primarily used for the affinity chromatography of His-tagged proteins. This product is based on our classic NUPharose agarose gel microspheres, pre-chelated with Co2+ from IMAC NUPharose FF , and is ready for direct use by the user. Different metal ions have varying affinity and specificity for target proteins: the affinity of Cu2+, Ni2+, Zn2+, and Co2+ decreases sequentially (affecting yield), while specificity increases sequentially (affecting purity). This product, chelated with Co2+, can be considered when Ni2 +-chelated resins fail to meet purity requirements. Compared to iminodiacetic acid (IDA) ligands, NTA ligands offer more balanced performance, better tolerance to chelating agents, reducing agents, alkali, etc., and exhibit lower metal ion leaching during the purification process.

IDA & Ni-IDA Metal Chelate Affinity Resin

Ni-IDA NUPharose FF and IDA NUPharose FF are metal chelate affinity chromatography resin containing iminodiacetic acid (IDA) groups, suitable for the affinity chromatography of most histidine-tagged (His-tag) proteins. Both products are based on our classic agarose gel microspheres, and “FF” stands for Fast Flow, indicating the resin can operate efficiently at high flow rates. IDA NUPharose FF is supplied without prechelated metal ions, allowing users to chelate metal ions such as Ni2+, Co2+, Cu2+, Zn2+ before use. Ni-IDA NUPharose FF is pre-chelated with Ni2+ and can be used directly. Through a unique ligand modification process, these products achieve a balance between affinity and specificity: binding capacity for most His-tagged proteins is greater than 40 mg/mL while achieving over 90% purity in a single-step purification.

r-Protein A Affinity Resin

The rProtein A ligand is expressed in E. coli, ensuring that it is free from animal-derived components while retaining all five binding domains of the wild-type protein. ProA NUPharose FF exhibits significantly higher binding capacity compared to wild-type Protein A resins, with a dynamic binding capacity for human IgG exceeding 40 mg/mL. This resin demonstrates affinity towards various types and subtypes of antibodies from multiple species, including human, mouse, rat, rabbit, and bovine sources. It is capable of isolating and purifying different subclasses of mammalian antibodies or genetically engineered recombinant proteins containing Fc fragments from ascites, serum, cell culture supernatants, or cell lysates, thereby meeting research and production requirements at various scales.